Journal
/
생화학
/
Ein optimierter Einzelmolekül-Pulldown-Assay zur Quantifizierung der Proteinphosphorylierung
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Please note that all translations are automatically generated. Click here for the English version.

Ein optimierter Einzelmolekül-Pulldown-Assay zur Quantifizierung der Proteinphosphorylierung

2,581 Views

•

07:45 min

•

June 06, 2022

DOI:

10.3791/63665-v

DOI:
10.3791/63665-v

•

07:45 min
June 06, 2022

•

6 Views
, , , , , , ,
1Department of Pathology,University of New Mexico School of Medicine, 2Department of Physics and Astronomy,University of New Mexico, 3Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Summary

Automatically generated

Das vorliegende Protokoll beschreibt die Probenvorbereitung und Datenanalyse zur Quantifizierung der Proteinphosphorylierung unter Verwendung eines verbesserten Einzelmolekül-Pulldown-Assays (SiMPull).

Explore More Videos

AI-powered Protein Phosphorylation QuantificationSingle-molecule Pull-down AssaySiMPull ProtocolAntibody LabelingAutofluorescence ReductionSingle-molecule AnalysisPhosphorylation Status Of Individual ProteinsAlternative To Western Blotting And Mass Spectrometry

Read Article

Cite this Article

Bailey, E. M., Salazar-Cavazos, E., Grattan, R. M., Wester, M. J., Schodt, D. J., Rojo, J. A., Lidke, K. A., Lidke, D. S. An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. J. Vis. Exp. (184), e63665, doi:10.3791/63665 (2022).

Download .ris file

Copy

Share Video

.

Copy