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An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

2,588 Views

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07:45 min

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June 06, 2022

DOI:

10.3791/63665-v

DOI:
10.3791/63665-v

•

07:45 min
June 06, 2022

•

6 Views
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1Patoloji Bölümü,University of New Mexico School of Medicine, 2Fizik ve Astronomi bölümü,New Mexico Üniversitesi, 3Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Özet

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The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

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Cite this Article

Bailey, E. M., Salazar-Cavazos, E., Grattan, R. M., Wester, M. J., Schodt, D. J., Rojo, J. A., Lidke, K. A., Lidke, D. S. An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation. J. Vis. Exp. (184), e63665, doi:10.3791/63665 (2022).

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