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Optisk rydding av plantevev for fluorescensavbildning
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JoVE 신문 생물학
Optical Clearing of Plant Tissues for Fluorescence Imaging

Optisk rydding av plantevev for fluorescensavbildning

DOI:
10.3791/63428-v

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04:55 min

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January 05, 2022

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, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Chapters

  • 00:05Introduction
  • 00:48Fixation of Samples
  • 01:48Clearing
  • 02:21Observation
  • 02:59Results: Optical Clearing and Fluorescence Imaging of Tissues
  • 04:18Conclusion

Summary

자동 번역

자동 번역

Her er en metode beskrevet for å gjøre plantevev gjennomsiktig samtidig som stabiliteten til fluorescerende proteiner opprettholdes. Denne teknikken letter dyp avbildning av ryddet plantevev uten fysisk seksjonering.

Tags

Optical ClearingFluorescence ImagingWhole-plant ImagingPlant-microbe InteractionsFixationFluorescent ProteinVacuum InfiltrationClearing SolutionClearSeePBSSilicone FrameFluorescent Microscopy

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