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Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes
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March 01, 2022
DOI:
1Division of Parasitic Diseases and Malaria, Entomology Branch,Centers for Disease Control and Prevention, 2Department of Entomology,Cornell University, 3Centro de Estudios en Biotecnologia,Universidad del Valle de Guatemala, 4Department of Cell Biology,Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute,Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology,Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology,Johns Hopkins School of Medicine, 8Biomedical Sciences Department,Idaho College of Osteopathic Medicine
Summary
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The oral administration of dsRNA produced by bacteria, a delivery method for RNA interference (RNAi) that is routinely used in Caenorhabditis elegans, was successfully applied here to adult mosquitoes. Our method allows for robust reverse genetics studies and transmission-blocking vector studies without the use of injection.
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Taracena, M., Hunt, C., Pennington, P., Andrew, D., Jacobs-Lorena, M., Dotson, E., Wells, M. Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes. J. Vis. Exp. (181), e63266, doi:10.3791/63266 (2022).
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