We illustrate the methods involved in screening and identification of the biosurfactant producing microbes. Methods for chromatographic characterization and chemical identification of the biosurfactants, determining the industrial applicability of the biosurfactant in enhancing residual oil recovery are also presented.
Nissar Zargar, A., Patil, N., Kumar, M., Srivastava, P. Enhanced Oil Recovery using a Combination of Biosurfactants. J. Vis. Exp. (184), e63207, doi:10.3791/63207 (2022).