Journal
/
면역학 및 감염병학
/
炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
Please note that all translations are automatically generated. Click here for the English version.

炎症性半月酶诱导的人单核细胞来源巨噬细胞邻近性的可视化

DOI:
10.3791/63162-v

•

08:41 min

•

April 06, 2022

•

,
1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 00:51Preparation of Cells for Electroporation
  • 02:11Nucleofection of Caspase BiFC Components into Human Monocyte-Derived Macrophages
  • 04:41Treatment of Transfected MDM and Caspase BiFC Data Acquisition
  • 06:37Results: Differentiation of CD14-Monocytes and Transfection of Human Monocyte-Derived Macrophages
  • 08:12Conclusion

Summary

자동 번역

자동 번역

该协议描述了从人血液样本中获取单核细胞来源巨噬细胞（MDM）的工作流程，一种在不损害细胞活力和行为的情况下将炎症半胱天冬酶双分子荧光互补（BiFC）报告基因有效引入人MDM的简单方法，以及一种基于成像的方法来测量活细胞中的炎症半胱天冬酶活化。

Tags

Inflammatory CaspasesProximityHuman Monocyte-derived MacrophagesVisualizationMolecular-levelPrimary Immune CellsOligomerizationMicroscopic MethodologiesTrypsin-EDTAPBSCell NumberHemocytometerReporter PlasmidCaspase BiFC PlasmidsNucleofectionElectroporation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

一个<em>在体外</em>共感染模型研究<em>恶性疟原虫</em人原发性单核细胞源性的免疫细胞-HIV-1相互作用

激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量

巨噬细胞集落刺激因子分化的人单核细胞衍生的巨噬细胞的培养

从全血人单核细胞来源的树突状细胞的生成

通过流式细胞术分析来自中枢神经系统的小胶质细胞和单核细胞衍生的巨噬细胞

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

单核细胞化学策略活性在Vivo的定量和血液单核细胞衍生巨噬细胞的特征

葡萄糖-6-磷酸异构酶（G6PI）诱导RA小鼠中iNKT细胞的采用免疫治疗

分化人类单细胞衍生巨噬细胞中细胞外陷阱释放的体外刺激与可视化

使用牛单核细胞来源的树突状细胞测定疫苗免疫原性

Read Article