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Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot
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Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

3,370 Views

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10:12 min

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October 28, 2021

DOI:

10.3791/63092-v

DOI:
10.3791/63092-v

•

10:12 min
October 28, 2021

•

4 Views
, , , ,
1Department of Medicine-Cardiology,University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics,University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine,University of Colorado Anschutz Medical Campus

Summary

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Detailed protocol and three Python scripts are provided for operating an open-source robotic liquid handling system to perform semi-automated protein sample preparation for mass spectrometry experiments, covering detergent removal, protein digestion, and peptide desalting steps.

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Shotgun ProteomicsAutomated Sample ProcessingOpen-source Lab RobotLiquid Handling SystemMass SpectrometryProtein BiochemistryProteomic StudiesAmmonium BicarbonateDTTTemperature ControlAutomationOpen-source Python Scripts

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Han, Y., Thomas, C. T., Wennersten, S. A., Lau, E., Lam, M. P. Y. Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot. J. Vis. Exp. (176), e63092, doi:10.3791/63092 (2021).

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