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黄色 ブドウ球菌 内在化と抗菌性化合物の細胞内有効性を研究するための酵素保護アッセイの改善

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JoVE 신문 생물학

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

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黄色ブドウ球菌内在化と抗菌性化合物の細胞内有効性を研究するための酵素保護アッセイの改善

2,456 Views

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06:36 min

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September 08, 2021

DOI:

10.3791/62903-v

DOI:

10.3791/62903-v

•

06:36 min

September 08, 2021

•

6 Views

Josselin Rigaill*^1,2, Estelle Audoux*¹, Killian Rodriguez¹, Aurélien Peyron¹, Philippe Berthelot^1,3, Jérôme Josse^4,5, Frédéric Laurent^4,5, Robin Caire¹, Paul O. Verhoeven^1,2

¹CIRI, Centre International de Recherche en Infectiologie, GIMAP team,University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, ²Department of Infectious Agents and Hygiene,University Hospital of St-Etienne, St-Etienne, France, ³Department of Infectious Diseases,University Hospital of St-Etienne, St-Etienne, France, ⁴CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team,University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, ⁵Department of Bacteriology, Institute for Infectious Agents,Hospices Civils de Lyon, Lyon, France

Summary

このプロトコルは、 黄色ブドウ球菌 の内在化の程度とヒト宿主細胞内で生き残る能力、ならびに抗菌性化合物の細胞内有効性を研究する方法を説明することを目的とする。

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