This protocol aims to describe how to study the extent of Staphylococcus aureus internalization and its ability to survive inside the human host cell, as well as the intracellular efficacy of antimicrobial compounds.
Rigaill, J., Audoux, E., Rodriguez, K., Peyron, A., Berthelot, P., Josse, J., Laurent, F., Caire, R., Verhoeven, P. O. Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds. J. Vis. Exp. (175), e62903, doi:10.3791/62903 (2021).