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Disección e inmunohistoquímica de la pierna adulta de Drosophila para detectar cambios en la unión neuromuscular de una neurona motora identificada
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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
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Disección e inmunohistoquímica de la pierna adulta de Drosophila para detectar cambios en la unión neuromuscular de una neurona motora identificada

3,870 Views

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11:02 min

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February 12, 2022

DOI:

10.3791/62844-v

DOI:
10.3791/62844-v

•

11:02 min
February 12, 2022

•

11 Views
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1Department of Biology,Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital,Warren Alpert Medical School of Brown University

Summary

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Describimos una técnica de disección que preserva la arquitectura de la unión neuromuscular y permite un estudio inmunocitoquímico detallado de las neuronas motoras en la pierna adulta de Drosophila .

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Stilwell, G., Agudelo, A. Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron. J. Vis. Exp. (180), e62844, doi:10.3791/62844 (2022).

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