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Biochemistry

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JoVE Journal Biochemistry
Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS)
 

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Article DOI: 10.3791/62732-v • 08:27 min • July 27th, 2021
July 27th, 2021 •
1, 1,2, 1,2
1Max-Planck-Institute of Molecular Plant Physiology, 2Center of Plant Systems Biology and Biotechnology

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요약
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In this protocol, we present an optimized workflow, which combines an efficient and fast sample preparation of many samples. In addition, we provide a step-by-step guide to reduce analytical variations for high-throughput evaluation of metabolic GWAS studies.

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Large-Scale Multi-Omics Genome-Wide Association Studies Mo-GWAS Guidelines Sample Preparation Normalization Machine-driven Variation Natural Diversity Technical Variation Integration Analysis Face Separation-based Extraction Metabolites Analytical Platform Systemic Error Biological Variance Genotypic Information Phenotypic Information Diverse Natural Population Kingdom Of Life Harvesting Tubes Metal Beads Homogenizing Label Tubes Liquid Nitrogen Fresh Leaf And Root Tissue Samples Flash Freezing 80 Degrees Celsius Storage Tube Holders Grinding Tissues Homogeneous Powder
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