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Regulación de células madre mesenquimales de la fagocitosis de macrófagos; Cuantificación e imágenes
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging
DOI:

09:10 min

July 16, 2021

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Chapters

  • 00:04Introduction
  • 00:50Mesenchymal Stem Cells Culture in Experimental Dish
  • 03:01Activation of MΦ (Macrophage Cells) with IFN-γ
  • 03:44MΦ (Macrophage Cells) Isolation and Co-culture with Mesenchymal Stem Cells
  • 04:53Zymosan Preparation and Addition to the Co-cultured Cells
  • 06:09Phagocytosis Assay and Dynamic Imaging
  • 07:57Results: Effect of IFN-γ on Phagocytosis
  • 08:44Conclusion

Summary

자동 번역

Aquí se presenta un protocolo para cuantificar y producir imágenes dinámicas de la regulación mediada por células madre mesenquimales (MSC) de la fagocitosis de macrófagos (MΦ) de partículas de levadura no opsonizada (zymosan) que se conjugan con una molécula fluorescente sensible al pH.

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