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צמיחה, טיהור, ו Titration של וירוס הרפס סימפלקס אונקוליטי
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
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צמיחה, טיהור, ו Titration של וירוס הרפס סימפלקס אונקוליטי

5,929 Views

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06:14 min

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May 13, 2021

DOI:

10.3791/62677-v

DOI:
10.3791/62677-v

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06:14 min
May 13, 2021

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7 Views
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1Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy,Texas Tech University Health Sciences Center, 2Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery,Massachusetts General Hospital and Harvard Medical School

Tags

Oncolytic Herpes Simplex VirusCancer ImmunotherapyVirus PropagationVirus PurificationVirus TitrationBiosafety Level TwoVirus StockAnticancer Immune ResponseVirus-based Cancer ImmunotherapyWild-type Herpes Simplex VirusGenetically Engineered Herpes Simplex VirusT-150 Square Centimeter FlasksDPBSIFCSDMEMCell ScraperCentrifugationVirus BufferBenzonase NucleaseVirus Titer

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Cite this Article

Nguyen, H., Sah, N., Humphrey, M. R. M., Rabkin, S. D., Saha, D. Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus. J. Vis. Exp. (171), e62677, doi:10.3791/62677 (2021).

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