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Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
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JoVE Journal 免疫学と感染
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

DOI:
10.3791/62677-v

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06:14 min

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May 13, 2021

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, , , ,
1Department of Immunotherapeutics and Biotechnology, Jerry H. Hodge School of Pharmacy,Texas Tech University Health Sciences Center, 2Molecular Neurosurgery Laboratory and the Brain Tumor Research Center, Department of Neurosurgery,Massachusetts General Hospital and Harvard Medical School

章

  • 00:04Introduction
  • 01:06Oncolytic Herpes Simplex Virus (oHSV) Growth
  • 02:46Oncolytic Herpes Simplex Virus (oHSV) Purification
  • 05:10Results: Microscopic Analysis of Vero Cells Infected with oHSV
  • 05:44Conclusion

概要

自動翻訳

自動翻訳

In this manuscript, we describe a simple method of growth, purification, and titration of the oncolytic herpes simplex virus for preclinical use.

タグ

Oncolytic Herpes Simplex VirusCancer ImmunotherapyVirus PropagationVirus PurificationVirus TitrationBiosafety Level TwoVirus StockAnticancer Immune ResponseVirus-based Cancer ImmunotherapyWild-type Herpes Simplex VirusGenetically Engineered Herpes Simplex VirusT-150 Square Centimeter FlasksDPBSIFCSDMEMCell ScraperCentrifugationVirus BufferBenzonase NucleaseVirus Titer

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