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Une méthode efficace pour l’induction de cellules dirigées de type hépatocytes à partir de cellules souches embryonnaires humaines
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JoVE 신문 면역학 및 감염병학
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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Une méthode efficace pour l’induction de cellules dirigées de type hépatocytes à partir de cellules souches embryonnaires humaines

2,593 Views

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08:05 min

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May 06, 2021

DOI:

10.3791/62654-v

DOI:
10.3791/62654-v

•

08:05 min
May 06, 2021

•

3 Views
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1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Summary

Automatically generated

Ce manuscrit décrit un protocole détaillé pour la différenciation des cellules souches embryonnaires humaines (CSEh) en cellules fonctionnelles de type hépatocytes (CHN) en complétant continuellement l’activine A et le CHIR99021 pendant la différenciation du CSEh en endoderme définitif (DE).

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Cite this Article

Zhou, Q., Xie, X., Zhong, Z., Sun, P., Zhou, X. An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells. J. Vis. Exp. (171), e62654, doi:10.3791/62654 (2021).

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