Visualization of Gut Microbiota-host Interactions via Fluorescence <em>In Situ</em> Hybridization, Lectin Staining, and Imaging

Katharine M. Ng; Carolina Tropini

doi:10.3791/62646

/

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

JoVE 신문
면역학 및 감염병학

This content is Free Access.

JoVE 신문 면역학 및 감염병학

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

6,513 Views

•

09:31 min

•

July 09, 2021

DOI:

10.3791/62646-v

DOI:

10.3791/62646-v

•

09:31 min

July 09, 2021

•

26 Views

Katharine M. Ng^1,2, Carolina Tropini^1,2,3

¹School of Biomedical Engineering,University of British Columbia, ²Department of Microbiology and Immunology,University of British Columbia, ³Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Summary

This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.

Explore More Videos

Gut Microbiota Host-associated Microbes Fluorescence In Situ Hybridization (FISH)Lectin Staining Imaging Host-microbiome Interface Methacarn Fixation Paraffin Embedding Intestinal Sections Visualization

Read Article

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Summary

Automatically generated

Explore More Videos