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Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

6,513 Views

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09:31 min

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July 09, 2021

DOI:

10.3791/62646-v

DOI:
10.3791/62646-v

•

09:31 min
July 09, 2021

•

26 Views
,
1School of Biomedical Engineering,University of British Columbia, 2Department of Microbiology and Immunology,University of British Columbia, 3Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Summary

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This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization.

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Ng, K. M., Tropini, C. Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging. J. Vis. Exp. (173), e62646, doi:10.3791/62646 (2021).

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