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低分子とdNTPase SAMHD1の相互作用を調べるためのハイスループット酵素共役活性アッセイ

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JoVE 신문 생화학

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

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低分子とdNTPase SAMHD1の相互作用を調べるためのハイスループット酵素共役活性アッセイ

1,854 Views

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08:17 min

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April 16, 2021

DOI:

10.3791/62503-v

DOI:

10.3791/62503-v

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08:17 min

April 16, 2021

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5 Views

Miriam Yagüe-Capilla, Sean G. Rudd

¹Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet

Tags

Enzyme-coupled Activity Assay SAMHD1 Small Molecule Interaction DNTPase Chemotherapy Drugs Hydrolysis Screening Assay Small Molecule Inhibitors Malachite Green Pyrophosphatase DGTP DGDP EDTA TCEP Tween-20

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