This content is Free Access.
A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1
1,942 Views
•
08:17 min
•
April 16, 2021
DOI:
Özet
Automatically generated
SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase with critical roles in human health and disease. Here we present a versatile enzyme-coupled SAMHD1 activity assay, deployed in a 384-well microplate format, that allows for the evaluation of small molecules and nucleotide analogues as SAMHD1 substrates, activators, and inhibitors.
Read Article
Cite this Article
Yagüe-Capilla, M., Rudd, S. G. A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1. J. Vis. Exp. (170), e62503, doi:10.3791/62503 (2021).
Copy