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纳米微分扫描荧光仪，用于在基于碎片的铅发现中筛选

JoVE 신문
생화학

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JoVE 신문 생화학

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

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纳米微分扫描荧光仪，用于在基于碎片的铅发现中筛选

4,229 Views

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06:26 min

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May 16, 2021

DOI:

10.3791/62469-v

DOI:

10.3791/62469-v

•

06:26 min

May 16, 2021

•

14 Views

Misbha Ud Din Ahmad¹, Alexander Fish¹, Jeroen Molenaar¹, Sridhar Sreeramulu², Christian Richter², Nadide Altincekic², Harald Schwalbe², Hans Wienk¹, Anastassis Perrakis¹

¹Oncode Institute and Division of Biochemistry,the Netherlands Cancer Institute, ²Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ),Johann Wolfgang Goethe-University

Summary

监测目标蛋白（即热移位检测、TSA）融化温度的变化是筛选几百种化合物片段库的有效方法。我们提出了一个TSA协议，实施机器人辅助纳米微分扫描荧光仪（nano-DSF），用于监测内在色氨酸荧光和光反散射，用于碎片筛选。

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