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Kapitel
- 00:04Introduction
- 00:35Plate Preparation
- 01:06Fragment Nano-Dispensing by the Mosquito Robot
- 02:01Fragment Dispensing
- 03:17Preparing the Prometheus
- 04:25Fluorescence Scan and Thermal Denaturation
- 05:04Results: Fragment Screening
- 05:49Conclusion
Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.
