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Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
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Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
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15:01 min
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September 18, 2021
DOI:
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases,Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry),Forschungszentrum Jülich
Summary
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We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.
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Cite this Article
Maslov, I. V., Bogorodskiy, A. O., Pavelchenko, M. V., Zykov, I. O., Troyanova, N. I., Borshchevskiy, V. I., Shevchenko, M. A. Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung. J. Vis. Exp. (175), e62436, doi:10.3791/62436 (2021).
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