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使用 D1S80 轨迹在实验室类中应用 DNA 指纹

JoVE 신문
유전학
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JoVE 신문 유전학

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

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使用 D1S80 轨迹在实验室类中应用 DNA 指纹

DOI:

10.3791/62305-v

•

08:35 min

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July 17, 2021

•

Meike Siebers*¹, Agatha Walla*^1,2, Thea Rütjes, Moritz-Fabian Müller, Maria von Korff^2,3

¹Institute of Plant Genetics,Heinrich-Heine-University, ²Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, ³Max Planck Institute for Plant Breeding Research, ⁴IBG-1: Biotechnology, Forschungszentrum Jülich GmbH

Chapters

00:00Introduction
01:03Step 1: DNA Template Preparation
03:18Step 2: Amplification of DIS80 VNTR Locus by PCR
04:02Step 3: Agarose Gel Electrophoresis of PCR Products
05:02Step 4: Analysis of Fragment Length Results
06:05Representative Results
06:39Conclusion

Summary

자동 번역

在这里，我们描述了一个简单的协议，通过放大VNTR轨迹 D1S80 从上皮细胞DNA生成DNA指纹配置文件。

Tags

DNA Fingerprinting D1S80 Locus Variable Number Of Tandem Repeats DNA Extraction PCR Gel Electrophoresis Buccal Mucosa Epithelial Cells Lyse Solution Denaturation Buffer Isopropanol Ethanol Elution Buffer Undergraduate Lab Class

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