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Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
JoVE Revista
Genética
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JoVE Revista Genética
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

DOI:
10.3791/62305-v

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08:35 min

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July 17, 2021

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, , , ,
1Institute of Plant Genetics,Heinrich-Heine-University, 2Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 3Max Planck Institute for Plant Breeding Research, 4IBG-1: Biotechnology, Forschungszentrum Jülich GmbH

Capítulos

  • 00:00Introduction
  • 01:03Step 1: DNA Template Preparation
  • 03:18Step 2: Amplification of DIS80 VNTR Locus by PCR
  • 04:02Step 3: Agarose Gel Electrophoresis of PCR Products
  • 05:02Step 4: Analysis of Fragment Length Results
  • 06:05Representative Results
  • 06:39Conclusion

Summary

Traducción Automática

Traducción Automática

Here we describe a simple protocol to generate DNA fingerprinting profiles by amplifying the VNTR locus D1S80 from epithelial cell DNA.

Tags

DNA FingerprintingD1S80 LocusVariable Number Of Tandem RepeatsDNA ExtractionPCRGel ElectrophoresisBuccal Mucosa Epithelial CellsLyse SolutionDenaturation BufferIsopropanolEthanolElution BufferUndergraduate Lab Class

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