NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Fragment-based screening by NMR is a robust method to rapidly identify small molecule binders to biomacromolecules (DNA, RNA, or proteins). Protocols describing automation-based sample preparation, NMR experiments & acquisition conditions, and analysis workflows are presented. The technique allows for optimal exploitation of both ¹H and ¹⁹F NMR-active nuclei for detection.