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Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease
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Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

2,561 Views

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05:51 min

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April 04, 2021

DOI:

10.3791/62244-v

DOI:
10.3791/62244-v

•

05:51 min
April 04, 2021

•

3 Views
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1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics,The Children’s Hospital of Philadelphia, 2Department of Pediatrics,University of Pennsylvania Perelman School of Medicine

Summary

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This study presents protocols for two semi-automated locomotor activity analysis approaches in C. elegans complex I disease gas-1(fc21) worms, namely, ZebraLab (a medium-throughput assay) and WormScan (a high-throughput assay) and provide comparative analysis among a wide array of research methods to quantify nematode behavior and integrated neuromuscular function.

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Caenorhabditis ElegansMitochondrial DiseaseLocomotor ActivityMotility AssaySemiautomated AssayLiquid MediumZebraLab SoftwareVideo RecordingActivity DetectionWorm BehaviorDrug Screening

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Cite this Article

Lavorato, M., Mathew, N. D., Shah, N., Nakamaru-Ogiso, E., Falk, M. J. Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease. J. Vis. Exp. (170), e62244, doi:10.3791/62244 (2021).

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