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scRNA-SeqおよびscATAC-Seqを用いた網膜遺伝子発現およびクロマチンアクセシビリティの多重化解析

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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scRNA-SeqおよびscATAC-Seqを用いた網膜遺伝子発現およびクロマチンアクセシビリティの多重化解析

3,319 Views

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06:24 min

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March 12, 2021

DOI:

10.3791/62239-v

DOI:

10.3791/62239-v

•

06:24 min

March 12, 2021

•

11 Views

Kurt Weir*¹, Patrick Leavey*¹, Clayton Santiago¹, Seth Blackshaw^1,2,3,4,5,6

¹Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, ²Department of Psychiatry and Behavioral Science,The Johns Hopkins Hospital, ³Department of Ophthalmology,The Johns Hopkins Hospital, ⁴Department of Neurology,The Johns Hopkins Hospital, ⁵Institute for Cell Engineering,Johns Hopkins University School of Medicine, ⁶Kavli Neuroscience Discovery Institute,Johns Hopkins University School of Medicine

Summary

ここで著者らは、網膜におけるトランスクリプトームおよびクロマチンアクセシビリティプロファイルを特徴付ける際の表現型決定およびその後のscRNA-seqおよびscATAC-seqのペアに対するMULTI-seqの有用性を示す。

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