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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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06:24 min
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March 12, 2021
DOI:
1Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, 2Department of Psychiatry and Behavioral Science,The Johns Hopkins Hospital, 3Department of Ophthalmology,The Johns Hopkins Hospital, 4Department of Neurology,The Johns Hopkins Hospital, 5Institute for Cell Engineering,Johns Hopkins University School of Medicine, 6Kavli Neuroscience Discovery Institute,Johns Hopkins University School of Medicine
Summary
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Here, the authors showcase the utility of MULTI-seq for phenotyping and subsequent paired scRNA-seq and scATAC-seq in characterizing the transcriptomic and chromatin accessibility profiles in retina.
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Cite this Article
Weir, K., Leavey, P., Santiago, C., Blackshaw, S. Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq. J. Vis. Exp. (169), e62239, doi:10.3791/62239 (2021).
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