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Journal / Immunology and Infection / Überwachung der NeutrophilenElastase- und…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Überwachung der NeutrophilenElastase- und Cathepsin-G-Aktivität in menschlichen Sputumproben
 
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Überwachung der NeutrophilenElastase- und Cathepsin-G-Aktivität in menschlichen Sputumproben

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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요약
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Die hier beschriebenen Protokolle bieten einen Leitfaden zur Visualisierung und Quantifizierung der Aktivität von Neutrophilenproteasen im menschlichen Sputum. Die Anwendungen einer solchen Analyse reichen von der Bewertung entzündungshemmender Behandlungen über die Validierung von Biomarkern, das Screening von Medikamenten bis hin zu klinischen Studien mit großen Kohorten.

Tags

Immunologie und Infektion Ausgabe 171 Mukoviszidose Lungenerkrankungen Entzündung Proteasen FRET-Reporter neutrophile Elastase Cathepsin G Diagnostik
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