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Immunology and Infection

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JoVE Journal Immunology and Infection
Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
 

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

Article DOI: 10.3791/62187-v • 09:26 min • January 22nd, 2021
January 22nd, 2021 •
*1, *1, 1,2, 1, 1,3, 1
1School of Life Science, University of Warwick, 2Institute of Microbiology and Infection, University of Birmingham, 3Warwick Antimicrobial Screening Facility, School of Life Science, University of Warwick
* These authors contributed equally

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요약
Automatic Translation

This workflow can be used to perform antibiotic susceptibility testing using an established ex vivo model of bacterial biofilm in the lungs of individuals with cystic fibrosis. Use of this model could enhance the clinical validity of MBEC (minimal biofilm eradication concentration) assays.

Tags

Antibiotic Efficacy Testing Ex Vivo Model Pseudomonas Aeruginosa Staphylococcus Aureus Biofilms Cystic Fibrosis Lung Bacteria Growth Biofilm Morphology Candidate Drugs Screening Antibiotic Susceptibility Testing EVPR Model Specialized Testing Individualized Testing Lungs From Meat Industry Ethical Concerns Sterilized Surface Bronchioles Tissue Sterilization
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