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斑马鱼光学杯形态形成的四维成像

JoVE 신문
신경과학

This content is Free Access.

JoVE 신문 신경과학

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

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斑马鱼光学杯形态形成的四维成像

3,210 Views

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07:26 min

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May 26, 2021

DOI:

10.3791/62155-v

DOI:

10.3791/62155-v

•

07:26 min

May 26, 2021

•

13 Views

Sarah Lusk¹, Macaulie A. Casey¹, Kristen M. Kwan¹

¹Department of Human Genetics,University of Utah

Summary

本协议描述了斑马鱼早期眼睛发育的托托标记和多维成像方法。我们使用激光扫描共聚焦显微镜描述标签、嵌入和四维（4D）成像，以及优化数据集获取以解剖光学杯形态发生机制的考虑因素。

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