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4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

4-Dimensional Imaging of Zebrafish Optic Cup Morphogenesis

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07:26 min

May 26, 2021

DOI:

07:26 min
May 26, 2021

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Summary

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This protocol describes an approach for in toto labeling and multidimensional imaging of zebrafish early eye development. We describe labeling, embedding, and four dimensional (4D) imaging using laser scanning confocal microscopy, and considerations for optimizing acquisition of datasets for dissecting mechanisms of optic cup morphogenesis.

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