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具有可移动XY阶段的平台孵化器:实现蛋白质细胞内快速光化学氧化的新平台
JoVE 신문
생화학
This content is Free Access.
JoVE 신문 생화학
Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
DOI:

05:50 min

May 17, 2021

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Chapters

  • 00:04Introduction
  • 00:45Setting Up the Platform Incubator for IC-FPOP
  • 02:08IC-FPOP in the Platform Incubator
  • 03:41Results: Analysis of Cell Transfection Efficiency, Proteins Modified in Single Cell Flow-System and PIXY, and Localization of IC-FPOP Modifications
  • 05:22Conclusion

Summary

자동 번역

一种新的静态平台用于利用一种称为蛋白质细胞内快速光化学氧化(IC-FPOP)的蛋白质足迹技术,对原生细胞环境中的蛋白质结构和相互作用部位进行特征。

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