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Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
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Bioquímica
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JoVE Revista Bioquímica
Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

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05:50 min

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May 17, 2021

DOI:

10.3791/62153-v

DOI:
10.3791/62153-v

•

05:50 min
May 17, 2021

•

1 Views
, , , ,
1Department of Pharmaceutical Sciences,University of Maryland Baltimore, 2Technology Research Center,University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology,University of Massachusetts

Tags

Platform IncubatorMovable XY StageIn-cell FPOPProtein-ligand InteractionsProtein-protein InteractionsConformational ChangesAutomated Six-well PlateCell CultureHydrogen PeroxideQuench BufferPeristaltic PumpsLaserCell ScraperCentrifugation

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Johnson, D., Punshon-Smith, B., Espino, J. A., Gershenson, A., Jones, L. M. Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins. J. Vis. Exp. (171), e62153, doi:10.3791/62153 (2021).

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