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JoVE Journal Neuroscience
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
 

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Article DOI: 10.3791/62127-v • 07:56 min • August 11th, 2021
August 11th, 2021 •
*1, *2, 3, 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine
* These authors contributed equally

챕터

요약
Automatic Translation

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

Tags

Keywords: Endogenous Monoamine Release Brain Slices Plate-based Assay Dorsal Striatum Hippocampus Efflux Buffer Pharmacological Conditions Vesicular Release Transporter-mediated Release
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