Journal
/
암 연구학
/
Creation and Maintenance of a Living Biobank – How We Do It
JoVE 신문
암 연구학
Author Produced
This content is Free Access.
JoVE 신문 암 연구학
Creation and Maintenance of a Living Biobank – How We Do It

Creation and Maintenance of a Living Biobank – How We Do It

DOI:
10.3791/62065-v

•

13:08 min

•

April 10, 2021

•

, , , , ,
1Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, University of Rostock, 2Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, 3Institute of Pathology,University Medical Center Rostock

Chapters

  • 00:00Introduction
  • 00:36Sample Acquisition
  • 02:41Tissue Processing
  • 05:43Cell Culture
  • 07:40Generation of Patient-Derived Xenografts
  • 11:16Explantation of PDX
  • 11:44Representative Results
  • 12:41Conclusion

Summary

자동 번역

자동 번역

In the following work, we describe the consecutive steps necessary for the establishment of a large biobank of colorectal and pancreatic cancer.

Tags

Living BiobankPatient-derived ModelsPrecision MedicineSample AcquisitionSerum ProcessingPBL IsolationSnap Freezing

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

A Mouse Model of Fatigue Induced by Peripheral Irradiation

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Read Article