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Journal / Genetics / CRISPR/Cas9 Editing of the C. elegans rbm-3.2…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
 

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Article DOI: 10.3791/62001-v • 07:46 min • December 11th, 2020
December 11th, 2020 •
1, *1, *1, *1, 1
1Department of Chemistry and Biochemistry, The University of Tulsa
* These authors contributed equally

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요약
Automatic Translation

The goal of this protocol is to enable seamless CRISPR/Cas9 editing of the C. elegans genome using assembled ribonucleoprotein complexes and the dpy-10 co-CRISPR marker for screening. This protocol can be used to make a variety of genetic modifications in C. elegans including insertions, deletions, gene replacements and codon substitutions.

Tags

CRISPR/Cas9 Editing C. Elegans Rbm-3.2 Gene Dpy-10 Co-CRISPR Screening Marker Ribonucleoprotein Complexes Genome Editing Microinjections Gonad Arms Positively Edited Worms Undergraduate Students MYOB Plate Incubation Injection Mix Nuclease-free Tubes Centrifugation Young Adult Worms
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