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1John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University School of Medicine, 2Graduate Program in Neuroscience,Washington University School of Medicine, 3Department of Neuroscience,Washington University School of Medicine, 4Hope Center for Neurological Disorders,Washington University School of Medicine
Chapters
- 00:05Introduction
- 00:42Imaging Preparation
- 03:44Two-Photon Image Acquisition
- 06:13Mouse Recovery
- 06:46Results: Representative In Vivo Mouse Retina Imaging
- 08:36Conclusion
In vivo imaging er et kraftig verktøy for studiet av biologi i helse og sykdom. Denne protokollen beskriver transpupillary avbildning av musens netthinne med et standard to-foton mikroskop. Det demonstrerer også forskjellige vivoimaging metoder for å fluorescerende merke flere cellulære kohorter av netthinnen.
