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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Chapters
- 00:05Introduction
- 01:14Macrophage Infection
- 02:07Infection Verification
- 03:04Sample Collection
- 03:47Results: Representative Effects of C. neoformans Macrophage Infection
- 05:04Conclusion
Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single experiment.
