The protocol described here aims to measure the hydrodynamic diameter of spherical nanoparticles, more specifically gold nanoparticles, in aqueous media by means of Nanoparticle Tracking Analysis (NTA). The latter involves tracking the movement of particles due to Brownian motion and implementing the Stokes-Einstein equation to obtain the hydrodynamic diameter.
Briffa, S. M., Sullivan, J., Siupa, A., Carnell-Morris, P., Carboni, M., Jurkschat, K., Peters, R. J. B., Schultz, C., Seol, K. H., Kwon, S., Park, S., Yoon, T. H., Johnston, C., Lofts, S., Valsami-Jones, E. Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison. J. Vis. Exp. (164), e61741, doi:10.3791/61741 (2020).