This article outlines the basic laboratory conditions and protocols for an incremental temperature regime to stimulate maximal spawning in the Mexican tetra Astyanax mexicanus, which is an emerging model for developmental and evolutionary studies.
Ma, L., Dessiatoun, R., Shi, J., Jeffery, W. R. Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus. J. Vis. Exp. (168), e61708, doi:10.3791/61708 (2021).