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Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging
Chapters
- 00:05Introduction
- 00:57Isolation of Deep Ectoderm Cells
- 03:09Generation of Mucociliary Epithelial Organoids
- 04:29High-resolution Live Imaging of Developing Organoids
- 05:23Results: Imaging of Developing Organoids
- 06:53Conclusion
We describe a simple protocol to develop mucociliary epithelial organoids from deep ectoderm cells isolated from Xenopus laevis embryos. The multipotent progenitors regenerate epithelial goblet cell precursors and allow live tracking of the initiation and progression of the cell transitions on the surface of organoids.
