Journal
/
면역학 및 감염병학
/
Konstruktion von Mutanten im Serotyp 1 Streptococcus pneumoniae Stamm 519/43
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Please note that all translations are automatically generated. Click here for the English version.

Konstruktion von Mutanten im Serotyp 1 Streptococcus pneumoniae Stamm 519/43

1,506 Views

•

06:06 min

•

September 11, 2020

DOI:

10.3791/61594-v

DOI:
10.3791/61594-v

•

06:06 min
September 11, 2020

•

2 Views
, , , ,
1Faculty of Infections and Tropical Diseases, Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Division of Medicine,University College London, 3Division of Infection and Immunity,University College London UCL

Summary

Automatically generated

Hier beschreiben wir einen S. pneumoniae Serotyp 1 Stamm 519/43, der genetisch verändert werden kann, indem er seine Fähigkeit nutzt, DNA und ein Suizid-Plasmid auf natürliche Weise zu erwerben. Als Prinzipienbeweis wurde eine isogene Mutante im Pneumolysin-Gen (Ply) hergestellt.

Explore More Videos

Serotype 1 Streptococcus PneumoniaeGenetic ModificationPlasmid ConstructionPSD1PSD2Spectinomycin CassetteRestriction DigestionLigationE. Coli TransformationS. Pneumoniae Culture

Read Article

Cite this Article

Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).

Download .ris file

Copy

Share Video

.

Copy