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Analyse de l’hypermutation somatique dans l’intron JH4 des cellules B du Centre Germinal à partir des patchs de Mouse Peyer
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
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Analyse de l’hypermutation somatique dans l’intron JH4 des cellules B du Centre Germinal à partir des patchs de Mouse Peyer

6,341 Views

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09:35 min

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April 20, 2021

DOI:

10.3791/61551-v

DOI:
10.3791/61551-v

•

09:35 min
April 20, 2021

•

6 Views
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1Department of Biology,The City College of New York and The Graduate Center of The City University of New York, 2Department of Microbiology,Icahn School of Medicine at Mount Sinai

Summary

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Présenté ici est un essai pour quantifier l’hypermutation somatique dans le locus de gène de chaîne lourde d’immunoglobuline utilisant les cellules b germinales de centre des corrections de Peyer de souris.

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Somatic HypermutationJH4 IntronGerminal Center B CellsPeyer’s PatchesAntibody DiversificationMolecular BiologyPCRSanger SequencingCell Isolation

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Cite this Article

Sible, E., Zheng, S., Choi, J. E., Vuong, B. Q. Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches. J. Vis. Exp. (170), e61551, doi:10.3791/61551 (2021).

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