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Het meten van natuurlijk verworven phagocytose-inducerende antilichamen tegen Plasmodium falciparum parasieten door een Flow Cytometrie-Gebaseerde Test
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences,University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology,University of Ghana, 3Department of Immunology,Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases,Rigshospitalet
Chapters
- 00:04Introduction
- 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
- 01:58Parasite Staining and Opsonization Plate Preparation
- 03:47THP-1 Cell and Phagocytosis Plate Preparation
- 04:35Phagocytosis Assay
- 06:21Flow Cytometry Acquisition and Analysis
- 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
- 09:21Conclusion
Het algemene doel van dit protocol is om instructies te geven over het meten van de capaciteit van antilichamen aanwezig in sera of plasma van individuen, van nature blootgesteld aan Plasmodium falciparum infectie, om fagocytose van de parasiet-geïnfecteerde erytrocyten (IEs) te opsoniseren en induceren.
