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Geautomatiseerde productie van menselijke geïnduceerde pluripotente stamcel-afgeleide corticale en dopaminerge neuronen met geïntegreerde live-cel monitoring
1Genome Biology of Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen
Chapters
- 00:05Introduction
- 00:43New Culture Plate and Tip Loading and Decontamination
- 01:35Culture Medium and/or Dissociation Reagent Filling
- 02:09Automated Method Execution
- 02:51Human Induced Pluripotent Stem Cell (hiPSC) Seeding
- 04:09Automated Confluence Assessment
- 04:47Medium Change
- 05:37Subcultivation
- 06:42Automated High Content, High Throughput Imaging
- 07:14Results: Representative Automated System-Produced hiPSC-Derived Cortical and Dopaminergic Neuron Characterization
- 08:58Conclusion
Tags
Automated ProductionHuman Induced Pluripotent Stem CellsCortical NeuronsDopaminergic NeuronsLive-cell MonitoringStandardized ProceduresLow Experimental VariationHigh Phenotypic ReproducibilityGraphic User InterfaceResource Instrument ProcessHEPA HoodDecontaminationUV RadiationLiquid Handling StationCulture MediumDissociation ReagentCell LineProjectBatchSeedingBrightfield Imaging CytometerCell Counting
