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نهج تفكك خلية واحدة للتحليل الجزيئي للمثانة البولية في الماوس بعد إصابة الحبل الشوكي
1Department of Urology,Boston Children’s Hospital, 2Department of Surgery,Harvard Medical School, 3Division of Hematology/Oncology,Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences,Harvard Medical School, 7Vascular Biology Program,Boston Children’s Hospital
Chapters
- 00:04Introduction
- 00:57Mouse Preparation
- 01:48Spinal Cord Injury (SCI)
- 03:17Tissue Perfusion and Procurement
- 04:34Cell Dissociation and Single Cell Suspension Preparation
- 05:59Cell Staining
- 06:44Flow Cytometry Analysis
- 07:43Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
- 08:54Conclusion
