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JoVE Journal Neuroscience
Isolering och kultur chick Ciliary Ganglion nervceller
 
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Isolering och kultur chick Ciliary Ganglion nervceller

Article DOI: 10.3791/61431-v • 14:36 min • August 8th, 2020
August 8th, 2020 •
*1, *1, 2, 2, 1,2
1Institute of Biomedicine, Department of Medical Sciences - iBiMED, University of Aveiro, 2CNC - Center for Neuroscience and Cell Biology, University of Coimbra
* These authors contributed equally

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Chick ciliary ganglier (CG) är en del av det parasympatiska nervsystemet. Neuronala kulturer av chick CG nervceller visade sig vara effektiva cellmodeller i studien av nervmuskelinteraktioner. Vi beskriver ett detaljerat protokoll för dissekering, dissociation och in vitro kultur CG nervceller från chick embryon.

Tags

Neurovetenskap Nummer 162 Cellkultur ciliary ganglion kyckling embryo dissekering parasympatiska nervceller neuromuskulära korsningar immuncytokemi fluorescensmikroskopi
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