Journal
/
생화학
/
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

3,784 Views

•

11:27 min

•

May 13, 2020

DOI:

10.3791/61288-v

DOI:
10.3791/61288-v

•

11:27 min
May 13, 2020

•

2 Views
, , ,
1Laboratory of Microbiology, Department of Molecular Biology,Université Libre de Bruxelles, 2Labiris Institut de Recherche

Summary

Automatically generated

This protocol has been developed to study the dimer-dodecamer transition of TmPep1050, an M42 aminopeptidase, at the structural level. It is a straightforward pipeline starting from protein purification to X-ray data processing. Crystallogenesis, data set indexation, and molecular replacement have been emphasized through a case of study, TmPep1050H60A H307A variant.

Explore More Videos

X-ray CrystallographyOligomeric StateThermotoga MaritimaAminopeptidaseTmPep1050Activity AssayApoenzyme PreparationDialysisUltrafiltration

Read Article

Cite this Article

Dutoit, R., Brandt, N., Van Elder, D., Droogmans, L. X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050. J. Vis. Exp. (159), e61288, doi:10.3791/61288 (2020).

Download .ris file

Copy

Share Video

.

Copy