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MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T
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10:22 min
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January 16, 2021
DOI:
1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science,Leiden University, 2Laboratory of Biophysics,Wageningen University & Research, 3Laboratory of BioNanoTechnology,Wageningen University & Research, 4MAGNEtic resonance Facility,Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre,Leiden University, 6Institute for Medical Physics and Biophysics,Leipzig University
Summary
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A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.
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van Schadewijk, R., Krug, J. R., Webb, A., Van As, H., Velders, A. H., de Groot, H. J. M., Alia, A. MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T. J. Vis. Exp. (167), e61266, doi:10.3791/61266 (2021).
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